The usage is idiomatic rather than rigidly grammatical. do is not needed.
Let alone = used to emphasize that something is more impossible than another thing:
You couldn't trust her to look after your dog, let alone your child
means “You would not trust her to look after your dog; it is even less likely that you would let her look after your child.”
Cambridge dictionary
Cambridge dictionary uses impossible but your example is better understood as improbable. “Few adults know Python; is is even less probable that children know it.” (This is the meaning of the sentence, regardless of any discussion of its truth)