Yes it is correct because the comma implies the conjunction. You actually don't even need the last comma at all, though sometimes it helps to clarify (most common example: let's eat, grandma vs let's eat grandma).
For your example:
He rides a bike, she drives a car, and I just walk.
would be expanded as
He rides a bike and she drives a car and I just walk.
(Note that with the use of the word 'just', 'but' would probably be a better conjunction: 'He rides a bike, she drives a car, but I just walk).
Examples for the other conjunctions:
He neither rides a bike, drives a car, nor walks. ('nor' would take place of the comma).
He wants to ride a bike, drive a car, or walk.
Has he ridden a bike, driven a car or walked yet?