Is "of" always supposed to be pronounced with the v sound (like "ov")? Or does it depend on the region (e.g. US, UK) or maybe on the word that follows the preposition?
For example, how would you pronounce the title of this question?
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The word of is often pronounced weakly, and the /v/ sound at the end of of is sometimes pronounced as [f]. The phrase “of course” is a typical example. I think that the /v/ in the word of is often pronounced as [f] before an unvoiced consonant.
Honestly speaking, this came as a surprise to me. As a foreign speaker, I learned the following “rule” at school: the word of in the phrase of course is pronounced with the “f” sound. (A similar “rule” is that the word have in have to is pronounced with the “f” sound.) Learning it as a rule had given me a (wrong) impression that English speakers are aware of it. However, while looking for a material to back up this “rule,” I learned that this is merely a variation of the actual sound of the same phoneme /v/.
According to the Longman Pronunciation Dictionary, the strong form is pronounced as [ɒv] (British) / [ʌv] (US), whereas the weak form is pronounced as [əv]. The informal short form, sometimes written as o', is pronounced as [ə]. There is no mentioning of any exceptions, suggesting that the of in "of course" (cf. other answers and comments) is pronounced in the same way (not with f). Audio files can be found here. The dictionary points out that "of" is a rare exception of a word where f is pronounced like v.