What I should use? Note that MOPSO and NSGA-II are two different methodologies.
Although MOPSO had not been applied to P300-BCI systems studies, NSGA-II did.
or
Although MOPSO had not been applied to P300-BCI systems studies, NSGA-II had.
What I should use? Note that MOPSO and NSGA-II are two different methodologies.
Although MOPSO had not been applied to P300-BCI systems studies, NSGA-II did.
or
Although MOPSO had not been applied to P300-BCI systems studies, NSGA-II had.
In the first part of your sentence, we are dealing with a pluperfect passive. The positive form is "had been [past participle]" and the negative form is "had not been [past participle]".
The auxiliary verb here is "had".
Therefore, the correct answer should be "had":
Although MOPSO had not been applied to P300-BCI systems studies, NSGA-II had.
Here is the sentence with the implied part included:
Although MOPSO had not been applied to P300-BCI systems studies, NSGA-II had been applied to the P300-BCI systems studies.