He is tired of that work
He is tired from that work.
I think the two sentences are almost the same.
If there is any difference, could anyone explain?
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"Tired of" suggests to me that the person has done enough of that particular work and does not wish to do any more. Perhaps that will change when they've had a break from it, or perhaps they no longer want to do it ever again, but in either case it's an expression of their attitude toward the work, not (necessarily) to how arduous the work is.
"Tired from" suggests that the work has been exhausting and their body or mind has become tired as a result of doing it, but that doesn't mean that they no longer enjoy the work.
Consider the example of chopping wood. Some people (myself included) enjoy a bit of good, hard physical labour that gets the blood flowing and builds the muscles. (Especially if you're in a white collar role and get to do it infrequently.) If I haven't done it for a while and am out of condition I find that I get tired FROM doing it much earlier than I would get tired OF doing it.
In other words "tired of" is more a question of attitude, "tired from" more a question of exertion.
"He is tired of that work" means, "He is tired of doing that type of work"; e. g., "He is tired of filing paperwork in folders all day, because it seems to him to be tedious and meaningless work."
"He is tired from that work" means, "He is tired from the physical difficulty of doing that work"; such as, "He is tired from lifting heavy pieces of concrete all day."