I am writing an email reply and want to confirm if the punctuation below is correct.
In response to your question, if it is available, my answer is “yes!"
EDIT: Also, should the exclamation mark be inside the the quotations or after.
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Without knowing what the question was, it's hard to see any justification for using an exclamation point. Replace it with a period. Exclamation points should be used very sparingly. Actually, when the words yes and no are used alone, that is, not as part of a longer quotation, you don't need to use quotation marks. In other words, you could write
. . . my answer is yes.